ABSTRACT
Objective To investigate the effect of fraxetin on primary cardiomyocyte hypertrophy induced by phenylephrine . Methods Primary SD cardiomyocyte hypertrophy was induced by phenylephrine ,and we observed the effects of fraxetin on cardio‐myocyte hypertrophy induced by phenylephrine .Image‐ProPlus 5 measured the area of cardiomyocyte;[3 H ]‐leucine incorporation assay detected the protein synthesis rate of cardiomyocyte;Real‐time PCR measured the Nrf2 and molecular markers (ANP ,BNP) mRNA expression levels of cardiomyyocyte hypertrophy .Results (1)Primary neonate SD Cardiomyocyte hypertrophy model was successfully established by 80 μmol/L phenylephrine for 48 h ,and Nrf2 expression levels significantly increased in cardiomyocyte hypertrophy model;(2)The increase of cardiomyocyte area ,protein synthesis rate and molecular markers expression of cardiomyyo‐cyte hypertrophy were significantly inhibited by fraxetin in a dose‐dependent manner .Conclusion Fraxetin could significantly inhib‐it the cardiomyyocyte hypertrophy induced by PE .
ABSTRACT
Objective To establish a method to isolate,culture and identify cardiomyocytes from neonatal mouse,estimate the cardiomyocytes survival rate,and identify the purity of cardiomyocytes.Methods Neonatal C5 7 mouse heart(within 3 days)was di-gested using trypsin and collagenase.Cardiomyocytes survival rate was estimated by trypan blue staining,and cardiomyocytes purity was identified byα-actin immunofluorescence staining.Results Mouse cardiomyocytes could be successfully isolated and cultured using neonatal mouse within 3 days.Trypan blue staining showed cardiomyocytes survival rate was>95%,and cardiomyocytes pu-rity was more than 95% demonstrated byα-actin immunofluorescence staining.Conclusion Under the strict experimental condi-tions,mouse cardiomyocytes can be successfully isolated and cultured with high survival rate and high purity.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the utilization of fuzzy matter - element model in evaluating the promoting effects of transdermal enhancers.</p><p><b>METHOD</b>Paracetamol was used as drug model. Azone, menthol, camphor, oleic acid, clove oil, Schizonepeta tenuifolia oil and patchouli were used as transdermal enhancers. Transdermal absorption experimentation of paracetamol was detected on the device of penetrating skins of rabbit in vitro and penetrating rates, steady fluxs, lag time and enhancement ratio were calculated to evaluate promoting effects of transdermal enhancers with fuzzy matter-element model based on variation coefficient weight.</p><p><b>RESULT</b>Paracetamol had the best promoting function with 2% clove oil and 2% camphor. The function drops slightly with 1% azone, 2% Schizonepeta tenuifolia oil, 2% menthol and 2% oleic acid ranked behind. The worst was with 2% patchouli.</p><p><b>CONCLUSION</b>Fuzzy matter-element model based on variation coefficient weight could judge the promoting and penetrating effect of transdermal enhancers objectively and feasible.</p>